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Nat Methods. 2013 Apr;10(4):347-53. doi: 10.1038/nmeth.2403. Epub 2013 Mar 10.

Quantitative estimation of activity and quality for collections of functional genetic elements.

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1
BIOFAB International Open Facility Advancing Biotechnology, Emeryville, California, USA.

Abstract

The practice of engineering biology now depends on the ad hoc reuse of genetic elements whose precise activities vary across changing contexts. Methods are lacking for researchers to affordably coordinate the quantification and analysis of part performance across varied environments, as needed to identify, evaluate and improve problematic part types. We developed an easy-to-use analysis of variance (ANOVA) framework for quantifying the performance of genetic elements. For proof of concept, we assembled and analyzed combinations of prokaryotic transcription and translation initiation elements in Escherichia coli. We determined how estimation of part activity relates to the number of unique element combinations tested, and we show how to estimate expected ensemble-wide part activity from just one or two measurements. We propose a new statistic, biomolecular part 'quality', for tracking quantitative variation in part performance across changing contexts.

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PMID:
23474467
DOI:
10.1038/nmeth.2403
[Indexed for MEDLINE]
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