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  • PMID: 23463721 was deleted because it is a duplicate of PMID: 23554380
Biotechnol Prog. 2013 May-Jun;29(3):825-8. doi: 10.1002/btpr.1717. Epub 2013 Apr 1.

Separation of nonfucosylated antibodies with immobilized FcγRIII receptors.

Author information

1
Process Biochemistry, Biogen Idec, Cambridge, MA, 02142, USA.

Abstract

Post-translational modifications can dramatically impact protein activity, but identifying such structure:function relationships, as well as capitalizing on functionally enhanced variants, is a significant challenge. Here, affinity chromatography resins that contained immobilized FcγRIII receptors were used to enrich nonfucosylated antibodies 6- to 9-fold, offering what may be a tractable method for both the identification of post-translational modifications that affect function, as well as a means to enrich variants with enhanced activity.

PMID:
23554380
DOI:
10.1002/btpr.1717
[Indexed for MEDLINE]

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