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Wei Sheng Wu Xue Bao. 2012 Dec 4;52(12):1458-66.

[Function of transporter genes fscTI and fscTII in the biosynthetic cluster of candicidin/FR-008].

[Article in Chinese]

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State Key Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200030, China.


OBJECTIVE To investigate function of transporter genes fscTI and fscTII in the biosynthetic gene cluster of candicidin/FR-008.


We constructed a plasmid pJTU4137 for disruption of transporter genes fscTI and fscTII by conjugation and homologous recombinant. The transporter genes were also PCR amplified and cloned into the high-copy plasmid pJTU1278 for overexpression in strain ZYJ-6 derived from Streptomyces sp. FR-008.


The disruption mutant LX10 was unable to produce candicidin and its analogues. Overexpression of FscTI and FscTII in ZYJ-6 caused a 1.5-fold increase in FR-008-III production compared with the control.


We confirmed that fscTI and fscTII are function as ATP dependent ATP binding cassetle (ABC) transporters in the biosynthetic gene cluster of FR-008. Furthermore, a positive example was provided for improving antibiotic production in other polyene producing strains based on the results that overexpression of fscTI and fscTI increased candicidin production.

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