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Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2013 Feb;38(2):120-4. doi: 10.3969/j.issn.1672-7347.2013.02.002.

[Construction of special reporter to detect DNA methylation regulatory activity in FCER1G gene promoter through patch-methylation].

[Article in Chinese]

Author information

1
Department of Dermatology, Central South University, Changsha, China.

Abstract

OBJECTIVE:

To construct a special luciferase reporter to detect DNA methylation regulatory activity in FCER1G gene promoter regulatory element.

METHODS:

We constructed special full and mock methylated FCER1G gene promoter regulatory luciferase reporters by patch-methylation, and detected DNA methylation regulatory activity by comparing the luciferase activity of full-methylated luciferase reporters with mock-methylated reporters.

RESULTS:

We successfully constructed the full and mock methylated FCER1G gene promoter regulatory luciferase reporters. The ratio of luciferase activity between the full methylated and the mock methylated was (0.36±0.07):1 (P<0.001).

CONCLUSION:

FCER1G promoter activity is methylation-sensitive and is regulated by DNA methylation.

[Indexed for MEDLINE]
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