Format

Send to

Choose Destination
Nat Methods. 2013 Apr;10(4):339-42. doi: 10.1038/nmeth.2397. Epub 2013 Mar 3.

A Y2H-seq approach defines the human protein methyltransferase interactome.

Author information

1
Otto-Warburg Laboratory, Max Planck Institute for Molecular Genetics (MPIMG), Berlin, Germany.

Abstract

To accelerate high-density interactome mapping, we developed a yeast two-hybrid interaction screening approach involving short-read second-generation sequencing (Y2H-seq) with improved sensitivity and a quantitative scoring readout allowing rapid interaction validation. We applied Y2H-seq to investigate enzymes involved in protein methylation, a largely unexplored post-translational modification. The reported network of 523 interactions involving 22 methyltransferases or demethylases is comprehensively annotated and validated through coimmunoprecipitation experiments and defines previously undiscovered cellular roles of nonhistone protein methylation.

PMID:
23455924
DOI:
10.1038/nmeth.2397
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center