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Nat Methods. 2013 Apr;10(4):307-14. doi: 10.1038/nmeth.2400. Epub 2013 Mar 3.

Interlaboratory reproducibility of large-scale human protein-complex analysis by standardized AP-MS.

Author information

1
Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.

Abstract

The characterization of all protein complexes of human cells under defined physiological conditions using affinity purification-mass spectrometry (AP-MS) is a highly desirable step in the quest to understand the phenotypic effects of genomic information. However, such a challenging goal has not yet been achieved, as it requires reproducibility of the experimental workflow and high data consistency across different studies and laboratories. We systematically investigated the reproducibility of a standardized AP-MS workflow by performing a rigorous interlaboratory comparative analysis of the interactomes of 32 human kinases. We show that it is possible to achieve high interlaboratory reproducibility of this standardized workflow despite differences in mass spectrometry configurations and subtle sample preparation-related variations and that combination of independent data sets improves the approach sensitivity, resulting in even more-detailed networks. Our analysis demonstrates the feasibility of obtaining a high-quality map of the human protein interactome with a multilaboratory project.

PMID:
23455922
DOI:
10.1038/nmeth.2400
[Indexed for MEDLINE]

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