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Curr Microbiol. 2013 Jul;67(1):100-6. doi: 10.1007/s00284-013-0334-5. Epub 2013 Feb 28.

Chromobacterium violaceum: important insights for virulence and biotechnological potential by exoproteomic studies.

Author information

1
Núcleo de Medicina Tropical, Universidade Federal do Pará, Av. Gen. Deodoro 92, Belém 66055-240, Brazil. alecip@gmail.com

Abstract

Chromobacterium violaceum is a beta-proteobacterium with high biotechnological potential, found in tropical environments. This bacterium causes opportunistic infections in both humans and animals, that can spread throughout several tissues, quickly leading to the death of the host. Genomic studies identified potential mechanisms of pathogenicity but no further studies were done to confirm the expression of these systems. In this study 36 unique protein entries were identified in databank from a two-dimensional profile of C. violaceum secreted proteins. Chromobacterium violaceum exoproteomic preliminary studies confirmed the production of proteins identified as virulence factors (such as a collagenase, flagellum proteins, metallopeptidases, and toxins), allowing us to better understand its pathogenicity mechanisms. Biotechnologically interesting proteins (such as chitinase and chitosanase) were also identified among the secreted proteins, as well as proteins involved in the transport and capture of amino acids, carbohydrates, and oxidative stress protection. Overall, the secreted proteins identified provide us important insights on pathogenicity mechanisms, biotechnological potential, and environment adaptation of C. violaceum.

PMID:
23455494
PMCID:
PMC3661913
DOI:
10.1007/s00284-013-0334-5
[Indexed for MEDLINE]
Free PMC Article

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