Equine colostral carbohydrates reduce lipopolysaccharide-induced inflammatory responses in equine peripheral blood mononuclear cells

Equine Vet J Suppl. 2012 Dec:(43):68-72. doi: 10.1111/j.2042-3306.2012.00680.x.

Abstract

Reasons for performing study: Increasing evidence suggests that reactions to lipopolysaccharide (LPS), particularly in the gut, can be partly or completely mitigated by colostrum- and milk-derived oligosaccharides. Confirmation of this hypothesis could lead to the development of new therapeutic concepts.

Objectives: To demonstrate the influence of equine colostral carbohydrates on the inflammatory response in an in vitro model with equine peripheral blood mononuclear cells (PBMCs).

Methods: Carbohydrates were extracted from mare colostrum, and then evaluated for their influence on LPS-induced inflammatory responses in PBMCs isolated from the same mares, mRNA expression of tumour necrosis factor-alpha, interleukin-6 and interleukin-10 was measured as well as the protein levels of tumour necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10).

Results: Equine colostral carbohydrates significantly reduced LPS-induced TNF-alpha protein at both times measured and significantly reduced LPS-induced TNF-alpha, IL-6 and IL-10 mRNA expression by PBMCs. Moreover, cell viability significantly increased in the presence of high concentrations of colostral carbohydrates.

Conclusions: Carbohydrates derived from equine colostrum reduce LPS-induced inflammatory responses of equine PBMCs.

Potential relevance: Colostrum and milk-derived carbohydrates are promising candidates for new concepts in preventive and regenerative medicine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carbohydrates / analysis
  • Carbohydrates / pharmacology*
  • Cell Survival / drug effects
  • Cells, Cultured
  • Colostrum / chemistry*
  • Female
  • Horses / blood*
  • Leukocytes, Mononuclear / drug effects*
  • Leukocytes, Mononuclear / metabolism*
  • Lipopolysaccharides / toxicity*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism

Substances

  • Carbohydrates
  • Lipopolysaccharides
  • RNA, Messenger