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Mol Biol Cell. 2013 Mar;24(5):535-42. doi: 10.1091/mbc.E12-09-0677.

Mapping and analysis of phosphorylation sites: a quick guide for cell biologists.

Author information

1
Department of Cell Biology, Harvard Medical School, Boston, MA 02115-5701, USA.

Abstract

A mechanistic understanding of signaling networks requires identification and analysis of phosphorylation sites. Mass spectrometry offers a rapid and highly sensitive approach to mapping phosphorylation sites. However, mass spectrometry has significant limitations that must be considered when planning to carry out phosphorylation-site mapping. Here we provide an overview of key information that should be taken into consideration before beginning phosphorylation-site analysis, as well as a step-by-step guide for carrying out successful experiments.

PMID:
23447708
PMCID:
PMC3583658
DOI:
10.1091/mbc.E12-09-0677
[Indexed for MEDLINE]
Free PMC Article

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