Format

Send to

Choose Destination
Metallomics. 2013 Apr;5(4):372-83. doi: 10.1039/c3mt20217h.

The BaeSR regulon is involved in defense against zinc toxicity in E. coli.

Author information

1
Department of Chemistry, The University of Michigan, 930 N University Ave, Ann Arbor, MI 48109, USA.

Abstract

Intracellular zinc homeostasis is regulated by an extensive network of transporters, ligands and transcription factors. The zinc detoxification functions of three transporters and a periplasmic protein regulated by the BaeSR two-component system were explored in this work by evaluating the effect of single gene knockouts in the BaeSR regulon on the cell growth rate, free zinc, total zinc and total copper after zinc shock. Two exporters, MdtABC and MdtD, and the periplasmic protein, Spy, are involved in zinc detoxification based on the growth defects at high cell density and increases in free (>1000-fold) and total zinc/copper (>2-fold) that were observed in the single knockout strains upon exposure to zinc. These proteins complement the ATP-driven zinc export mediated by ZntA in E. coli to limit zinc toxicity. These results highlight the functions of the BaeSR regulon in metal homeostasis.

PMID:
23446818
PMCID:
PMC3657296
DOI:
10.1039/c3mt20217h
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Royal Society of Chemistry Icon for PubMed Central
Loading ...
Support Center