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Development. 2013 Mar;140(6):1364-8. doi: 10.1242/dev.091844.

ClearT: a detergent- and solvent-free clearing method for neuronal and non-neuronal tissue.

Author information

1
Department of Pathology and Cell Biology, Columbia University, College of Physicians and Surgeons, 630 West 168th Street, 14-509 P&S, New York, NY 10032, USA.

Abstract

We describe a clearing method for enhanced visualization of cell morphology and connections in neuronal and non-neuronal tissue. Using Clear(T) or Clear(T2), which are composed of formamide or formamide/polyethylene glycol, respectively, embryos, whole mounts and thick brain sections can be rapidly cleared with minimal volume changes. Unlike other available clearing techniques, these methods do not use detergents or solvents, and thus preserve lipophilic dyes, fluorescent tracers and immunohistochemical labeling, as well as fluorescent-protein labeling.

PMID:
23444362
PMCID:
PMC3912244
DOI:
10.1242/dev.091844
[Indexed for MEDLINE]
Free PMC Article

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