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Brain Inj. 2013;27(3):354-60. doi: 10.3109/02699052.2012.743184.

Effect of neuroprotective therapies (hypothermia and cyclosporine a) on dopamine-induced apoptosis in human neuronal SH-SY5Y cells.

Author information

1
Department of Critical Care Medicine, University Hospital Marqués de Valdecilla-IFIMAV, Santander, Spain. gelesballesteros@yahoo.es

Abstract

INTRODUCTION:

This study aimed to evaluate the effect of hypothermia and CyA on neuronal survival after induced injury in a neuronal model.

METHODS:

Human neuroblastoma SH-SY5Y cells were seeded and allowed to grow. To determine whether lower temperatures protect from dopamine-induced apoptosis, cells were treated with dopamine at 100 µM, at 300 µM or without dopamine and incubated at 32 °C or 37 °C for 24 hours. To assess the effect of CyA, cells were pre-incubated with CyA at 37 °C and after dopamine was added.

RESULTS:

After 24 hours of incubation at 37 °C, 100 µM and 300 µM dopamine induced 42% (SD = 21) and 58% (SD = 7.9) apoptotic SH-SY5 cells, respectively. In cultures at 32 °C dopamine-induced apoptosis could be reversed by hypothermia [7% (SD = 1.4) and 3.45% (SD = 1.1) for 100 µM and 300 µM, respectively], similar to levels obtained in non-treated cells [2.4% (SD = 1.5)]. Cyclosporine A treatment did not render the expected result, since CyA-pre-treated cells and SH-SY5Y cells showed higher levels of apoptosis than those observed with dopamine alone

CONCLUSIONS:

Hypothermia has a marked protective effect against apoptotic cell death induced by dopamine in a human neuroblastic cell line. The neuroprotective effect of CyA described with other apoptotic cell death stimuli was not demonstrated with our experimental conditions.

PMID:
23438355
DOI:
10.3109/02699052.2012.743184
[Indexed for MEDLINE]

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