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Nat Methods. 2013 Apr;10(4):332-4. doi: 10.1038/nmeth.2378. Epub 2013 Feb 24.

Neutron-encoded mass signatures for multiplexed proteome quantification.

Author information

1
Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.

Abstract

We describe a protein quantification method called neutron encoding that exploits the subtle mass differences caused by nuclear binding energy variation in stable isotopes. These mass differences are synthetically encoded into amino acids and incorporated into yeast and mouse proteins via metabolic labeling. Mass spectrometry analysis with high mass resolution (>200,000) reveals the isotopologue-embedded peptide signals, permitting quantification. Neutron encoding will enable highly multiplexed proteome analysis with excellent dynamic range and accuracy.

PMID:
23435260
PMCID:
PMC3612390
DOI:
10.1038/nmeth.2378
[Indexed for MEDLINE]
Free PMC Article

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