Format

Send to

Choose Destination
See comment in PubMed Commons below
Analyst. 2013 Apr 7;138(7):2068-72. doi: 10.1039/c3an36634k. Epub 2013 Feb 25.

A ratiometric fluorescent probe for hydrophobic proteins in aqueous solution based on aggregation-induced emission.

Author information

1
Department of Chemistry, Tsinghua University, Beijing 100084, PR China.

Abstract

A novel fluorescent probe 1 is reported here with ratiometric response to hydrophobic proteins (casein) or proteins with hydrophobic pockets (BSA, HSA) through hydrophobic interaction. Probe 1 underwent deprotonation in aqueous solution at pH 7.4 and emitted blue fluorescence at 436 nm. Upon the addition of BSA, HSA or casein, the aggregation-induced emission fluorescence of 1 at 518 nm was turned on. The fluorescence intensity ratio, I518/I436 was linearly related to the concentrations of these proteins. The detection limits for BSA, HSA and casein based on IUPAC (CDL = 3Sb m(-1)) were 16.2 μg mL(-1), 10.5 μg mL(-1) and 5.7 μg mL(-1), respectively.

PMID:
23435163
DOI:
10.1039/c3an36634k
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Royal Society of Chemistry
    Loading ...
    Support Center