CD24+ adipocyte progenitors give rise to CD24− cells in vivo. (a) Flow cytometry plots of SVF from a time course SWAT development in C57BL/6 mice at the indicated embryonic (e) and post-natal (P) days. Dot plots show Lin−:CD29+:CD34+ cells. (b) CD24 staining in Lin−:CD29+:CD34+:Sca1+ cells from e17.5 and P42 SWAT. (c) Oil Red O staining of differentiated FACS sorted cell populations from e17.5 SWAT. Scale bar represents 100 μm. (d) Representative images of flow cytometry plots of SWAT Lin−:CD29+:CD34+ cells from 6-week old Rag2-/-;IL2Rγ-/-;A-Zip mice at indicated days post transplantation of 100,000 FACS-isolated dTomato+, Lin−:CD29+:CD34+:Sca-1+:CD24+ (CD24+) cells from 6-week old mT/mG mice. Plots show overlay of dTomato+ transplanted cells (white) on Rag2-/-;IL2Rγ-/-;A-Zip recipient SVF cells (blue). (e) Quantification of flow cytometry analysis of dTomato+ CD24+ transplantations into Rag2-/-;IL2Rγ-/-;A-Zip mice (n=4 transplantations, **** p<0.0001, error bar represent S.E.M.). (f). Representative flow cytometry plot of SWAT Lin−:CD29+:CD34+ cells from 6-week old Rag2-/-;IL2Rγ-/-;A-Zip mice at 1 day post transplantation of 100,000 FACS isolated dTomato+, CD24− cells from 6-week old mT/mG mice. The dot plot shows overlay of dTomato+ transplanted cells (white) on Rag2-/-;IL2Rγ-/-;A-Zip recipient SVF cells (blue). (g) Quantification of flow cytometry analysis of dTomato+ CD24− transplantations into Rag2-/- ;IL2Rγ-/-;A-Zip mice (n=3 transplants, error bars represent S.E.M.).