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Dev Cell. 2013 Mar 11;24(5):517-29. doi: 10.1016/j.devcel.2013.01.015. Epub 2013 Feb 21.

Removal of retained introns regulates translation in the rapidly developing gametophyte of Marsilea vestita.

Author information

1
University of Maryland at College Park, Department of Cell Biology and Molecular Genetics, College Park, MD 20742, USA.

Abstract

The utilization of stored RNA is a driving force in rapid development. Here, we show that retention and subsequent removal of introns from pre-mRNAs regulate temporal patterns of translation during rapid and posttranscriptionally controlled spermatogenesis of the fern Marsilea vestita. Analysis of RNAseq-derived transcriptomes revealed a large subset of intron-retaining transcripts (IRTs) that encode proteins essential for gamete development. Genomic and IRT sequence comparisons show that other introns have been previously removed from the IRT pre-mRNAs. Fully spliced isoforms appear at distinct times during development in a spliceosome-dependent and transcription-independent manner. RNA interference knockdowns of 17/17 IRTs produced anomalies after the time points when those transcripts would normally be spliced. Intron retention is a functional mechanism for forestalling precocious translation of transcripts in the male gametophyte of M. vestita. These results have broad implications for plant gene regulation, where intron retention is widespread.

PMID:
23434411
DOI:
10.1016/j.devcel.2013.01.015
[Indexed for MEDLINE]
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