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Curr Pharm Des. 2013;19(30):5443-8.

SNAP-tag as a tool for surface immobilization.

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1
Karlsruhe Institute of Technology (KIT), DFG-Center for Functional Nanostructures (CFN), Wolfgang Gaede Str. 1, 76131 Karlsruhe, Germany.

Abstract

SNAP-tag technology has been an important tool for protein study for more than a decade and in the meanwhile has found a number of applications beyond the field of molecular biology and protein purification. Based on covalent interaction of SNAP-tag, 20 kDA mutant of DNA repair protein and benzylguanine, it enables irreversible and controllable protein modification. In this mini review, recent developments in the use of SNAP-tag for the design of protein arrays and nanoparticle biofunctionalization are presented and discussed. A short overview of other applications that paved the way to surface modifications is also given with emphasis on fluorescent labeling through the use of SNAP-tag fusion proteins. Finally, the future of the SNAP-tag methodology for surface patterning and 3D structural scaffolding is addressed.

PMID:
23431987
[Indexed for MEDLINE]
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