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Infect Immun. 2013 May;81(5):1382-9. doi: 10.1128/IAI.00044-13. Epub 2013 Feb 19.

The degree of Helicobacter pylori-triggered inflammation is manipulated by preinfection host microbiota.

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1
Departments of Molecular, Cell, and Developmental Biology, University of California, Santa Cruz, Santa Cruz, California, USA.

Abstract

Helicobacter pylori infects over 3 billion people worldwide and is the primary risk factor for gastric cancer. Most individuals infected with H. pylori develop only asymptomatic gastritis; however, some develop ulcers or gastric adenocarcinoma. We demonstrate that one previously unappreciated parameter influencing H. pylori disease outcome is variation in the preinfection host microbiota. Utilizing a mouse model, we altered the microbiota by antibiotic treatment and found that these alterations resulted in significantly lowered H. pylori-triggered inflammation. Specifically, antibiotic pretreatment reduced CD4(+) T-helper cells and Ifnγ transcript levels in gastric tissue after H. pylori infection. The bacterial communities in mice with a reduced response to H. pylori displayed many differences from those in untreated mice, including significantly more cluster IV and XIVa Clostridium spp., bacteria known to influence inflammation via regulatory T cell populations. Our findings suggest that microbiota composition, perhaps Clostridium spp., contributes to the variable disease outcome of H. pylori infection by altering the recruitment of CD4(+) T cells to the gastric compartment. Our results suggest that gastric microbiota could be used as a diagnostic tool to determine which patients are at risk for developing severe disease.

PMID:
23429529
PMCID:
PMC3647981
DOI:
10.1128/IAI.00044-13
[Indexed for MEDLINE]
Free PMC Article
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