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J Clin Microbiol. 2013 May;51(5):1578-9. doi: 10.1128/JCM.03428-12. Epub 2013 Feb 20.

Magnetic bead protocol for culturing Mycobacterium tuberculosis from sputum specimens.

Author information

1
Aix Marseille Université, URMITE, UMR CNRS 7278, IRD 198, Inserm 1095, Marseille, France.

Abstract

A magnetic bead protocol and a standard centrifugation protocol yielded Mycobacterium tuberculosis in 40/50 sputum specimens in 12 ± 1 days and 11 ± 2 days, respectively (P > 0.05 by Student's t test). Manipulation took 35 ± 5 min and 45 ± 10 min, respectively (P < 0.05). The magnetic bead protocol could advantageously replace centrifugation for culturing M. tuberculosis from sputum.

PMID:
23426921
PMCID:
PMC3647907
DOI:
10.1128/JCM.03428-12
[Indexed for MEDLINE]
Free PMC Article

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