Quantitative imaging of a therapeutic peptide in biological tissue sections by MALDI MS

Bioanalysis. 2013 Mar;5(5):603-12. doi: 10.4155/bio.13.13.

Abstract

Background: Therapeutic peptides and proteins are being increasingly explored as potential therapeutic agents for molecular-targeted therapy, and the requirement for quantitative bioanalytical tools for such molecules has been discussed.

Results: The distribution of octreotide, a synthetic octapeptide analog of somatostatin, in the liver and kidney of mice administered with the analog was clearly visualized by MALDI-Imaging MS (IMS). The developed MALDI-IMS analytical method successfully quantified the amount of octreotide on tissue sections (accuracy was 76-127%) and the 2,5-dihydroxybenzoic acid-based normalization method was effective.

Conclusion: The results of this study suggest that MALDI-IMS enables the quantification of an administered therapeutic peptide on biological tissue sections, as well as visualization of the in vivo distribution of the peptide.

MeSH terms

  • Animals
  • Chromatography, High Pressure Liquid
  • Gelatin / chemistry
  • Gentisates / chemistry
  • Kidney / metabolism
  • Kidney / pathology
  • Liver / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Octreotide / analysis
  • Peptides / analysis*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*

Substances

  • Gentisates
  • Peptides
  • Gelatin
  • Octreotide
  • 2,5-dihydroxybenzoic acid