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Genesis. 2013 Jun;51(6):430-5. doi: 10.1002/dvg.22381. Epub 2013 Mar 28.

Generation and characterization of KsprtTA and KsptTA transgenic mice.

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1
Department of Internal Medicine (Nephrology), UT Southwestern Medical Center, Dallas, Texas 75390-9148, USA.

Abstract

The advent of technologies that allow tissue specific expression or ablation of genes has contributed enormously to our knowledge of the mechanism regulating organ development and maintenance in mice. The tetracycline inducible system allows reversible regulation of gene products upon administration of doxycycline. Here we describe the generation and activity of two transgenic lines expressing the cDNAs for the Tet responsive transcription factors rtTA and tTA (Tet-on and off) respectively under the control of an element that drives expression in the epithelium of the developing and adult kidney. Both lines show inducible and reversible activity in the embryonic and adult organ.

PMID:
23420736
PMCID:
PMC3696052
DOI:
10.1002/dvg.22381
[Indexed for MEDLINE]
Free PMC Article
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