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Nat Methods. 2013 Mar;10(3):246-8. doi: 10.1038/nmeth.2372. Epub 2013 Feb 17.

Single-chromosome transcriptional profiling reveals chromosomal gene expression regulation.

Author information

1
Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania, USA.

Erratum in

  • Nat Methods. 2013 May;10(5):445.

Abstract

We report intron chromosomal expression FISH (iceFISH), a multiplex imaging method for measuring gene expression and chromosome structure simultaneously on single chromosomes. We find substantial differences in transcriptional frequency between genes on a translocated chromosome and the same genes in their normal chromosomal context in the same cell. Correlations between genes on a single chromosome pointed toward a cis chromosome-level transcriptional interaction spanning 14.3 megabases.

PMID:
23416756
PMCID:
PMC4131260
DOI:
10.1038/nmeth.2372
[Indexed for MEDLINE]
Free PMC Article

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