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FEBS Lett. 2013 Mar 18;587(6):614-9. doi: 10.1016/j.febslet.2013.01.073. Epub 2013 Feb 14.

DksA2, a zinc-independent structural analog of the transcription factor DksA.

Author information

1
Department of Microbiology, The Ohio State University, Columbus, OH, USA.

Abstract

Transcription factor DksA contains a four-Cys Zn(2 +)-finger motif thought to be responsible for structural integrity and the relative disposition of its domains. Pseudomonas aeruginosa encodes an additional DksA paralog (DksA2) that is expressed selectively under Zn(2+) limitation. Although DksA2 does not bind Zn(2+), it complements the Escherichia coli dksA deletion and has similar effects on transcription in vitro. In this study, structural and biochemical analyses reveal that DksA2 has a similar fold, domain structure and RNA polymerase binding properties to those of the E. coli DksA despite the lack of the stabilizing metal ion.

PMID:
23416301
PMCID:
PMC5525025
DOI:
10.1016/j.febslet.2013.01.073
[Indexed for MEDLINE]
Free PMC Article

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