Format

Send to

Choose Destination
See comment in PubMed Commons below
Anal Biochem. 2013 May 15;436(2):187-9. doi: 10.1016/j.ab.2013.01.033. Epub 2013 Feb 12.

HPLC-MTT assay: anticancer activity of aqueous garlic extract is from allicin.

Author information

1
Illinois Mathematics and Science Academy, Aurora, IL 60506, USA.

Abstract

A strategy using reversed-phase high-performance liquid chromatography (HPLC), thin layer chromatography (TLC), mass spectrometry (MS), nuclear magnetic resonance (NMR), chemical synthesis, and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay to identify allicin as the active anticancer compound in aqueous garlic extract (AGE) is described. Changing the pH of AGE from 7.0 to 5.0 eliminated interfering molecules and enabled a clean HPLC separation of the constituents in AGE. MTT assay of the HPLC fractions identified an active fraction. Further analysis by TLC, MS, and NMR verified the active HPLC fraction as allicin. Chemically synthesized allicin was used to provide further confirmation. The results clearly identify the active compound in AGE as allicin.

PMID:
23416182
DOI:
10.1016/j.ab.2013.01.033
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center