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Bioanalysis. 2013 Feb;5(4):449-62. doi: 10.4155/bio.12.337.

Capillary microsampling and analysis of 4-µl blood, plasma and serum samples to determine human α-synuclein elimination rate in mice.

Author information

1
Global DMPK, In Vivo Screening & Profiling, AstraZeneca R&D, SE-15185, Södertälje, Sweden. ovex.jonsson@gmail.com

Abstract

BACKGROUND:

The capillary microsampling technique was scaled down to enable repeated PK sampling of blood, plasma and serum from mice for the determination of the 14-kDa protein α-synuclein using the Gyrolab™ immunoassay platform.

RESULTS:

4-µl plasma, serum or blood samples were taken from 36 mice, in total 648 samples were successfully collected and analyzed. Following intravenous administration of human α-synuclein to mice, the elimination of α-synuclein was rapid, with a half-life in plasma of 1.1 h. High endogenous levels in red blood cells in combination with some hemolysis led to a challenge in the evaluation of α-synuclein exposure in plasma and serum.

CONCLUSION:

The small sample volumes and flexibility in choice of liquid matrices using the capillary microsampling technique enable repeated sampling in mouse studies, as well as multi-matrix analysis if needed. Liquid microsampling is well suited for micro- and nano-liter scale immunoassays.

PMID:
23414378
DOI:
10.4155/bio.12.337
[Indexed for MEDLINE]

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