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Langmuir. 2013 Jun 18;29(24):7369-76. doi: 10.1021/la304548m. Epub 2013 Mar 19.

High-performance surface acoustic wave immunosensing system on a PEG/aptamer hybridized surface.

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1
Graduate School of Pure and Applied Sciences, University of Tsukuba, Tsukuba, Japan.

Abstract

Label-free immunoassay systems have the advantages of procedural simplicity and a low construction cost of surfaces for immunosensing. When label-free immunoassay systems are considered, the nonspecific adsorption of unwanted materials should be eliminated unless it aids in the detection of error. PEG is well-known as a blocking agent for the prevention of the adsorption of nonspecific binding materials when coimmobilized with ligands for targets such as antibodies and oligonucleotides. The construction strategy for PEG/ligand coimmobilized surfaces is an important point in the preparation of a high-performance assays because the physiological condition of the ligand depends strongly on its interaction with the PEG chain. In this report, we investigate the interaction between thrombin and a thrombin-binding aptamer (TBA) on a PEG/TBA coimmobilized surface by using a shear horizontal surface acoustic wave (SAW) sensor. The thrombin-TBA binding property shows remarkable differences with changes in the PEG density and the distance from the gold surface to the aptamer.

PMID:
23414210
DOI:
10.1021/la304548m
[Indexed for MEDLINE]
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