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Bioprocess Biosyst Eng. 2013 Nov;36(11):1797-801. doi: 10.1007/s00449-013-0922-6. Epub 2013 Feb 15.

A two-stage cultivation process for the growth enhancement of Chlorella vulgaris.

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Department of Chemical and Materials Engineering, Tunghai University, Taiwan, Taiwan, ROC,


This study proposes a two-stage cultivation process with an autotrophic growth followed by a mixotrophic process. The results indicated that a two-stage cultivation process using a daily dose of 3 g/L of glucose could achieve 7.4 g/L of biomass, which was about a 64 % increase over simple autotrophic cultivation. In the second stage of mixotrophic cultivation, glucose was regarded as a better carbon source for cell growth, than was glycerol. Linoleic acid (C18:2) would be the primary component in the two-stage cultivation as in the autotrophic cultivation. Even carbon source was provided in the second stage of mixotrophic cultivation; lower light intensity limited the mixotrophic growth, which indicated that photosynthesis still plays an important role in the second stage of mixotrophical cultivation. The final biomass was higher after this two-stage cultivation process, which made it suitable for application in the production scale-up of algal biomass.

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