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Am J Obstet Gynecol. 2013 May;208(5):399.e1-8. doi: 10.1016/j.ajog.2013.02.018. Epub 2013 Feb 11.

Modulation of lipopolysaccharide-induced chorioamnionitis by Ureaplasma parvum in sheep.

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  • 1Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, University of Cincinnati College of Medicine, Cincinnati, OH, USA. .

Abstract

OBJECTIVE:

Ureaplasma colonization in the setting of polymicrobial flora is common in women with chorioamnionitis, and is a risk factor for preterm delivery and neonatal morbidity. We hypothesized that Ureaplasma colonization of amniotic fluid would modulate chorioamnionitis induced by Escherichia coli lipopolysaccharide (LPS).

STUDY DESIGN:

Sheep received intraamniotic (IA) injections of media (control) or live Ureaplasma either 7 or 70 days before delivery. Another group received IA LPS 2 days before delivery. To test for interactions, U parvum-exposed animals were challenged with IA LPS, and delivered 2 days later. All animals were delivered preterm at 125 ± 1 day of gestation.

RESULTS:

Both IA Ureaplasma and LPS induced leukocyte infiltration of chorioamnion. LPS greatly increased the expression of proinflammatory cytokines and myeloperoxidase in leukocytes, while Ureaplasma alone caused modest responses. Interestingly, 7-day but not 70-day Ureaplasma exposure significantly down-regulated LPS-induced proinflammatory cytokines and myeloperoxidase expression in the chorioamnion.

CONCLUSION:

Acute (7-day) U parvum exposure can suppress LPS-induced chorioamnionitis.

PMID:
23410690
PMCID:
PMC3637868
DOI:
10.1016/j.ajog.2013.02.018
[PubMed - indexed for MEDLINE]
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