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PLoS One. 2013;8(2):e55725. doi: 10.1371/journal.pone.0055725. Epub 2013 Feb 7.

eIF4GI facilitates the MicroRNA-mediated gene silencing.

Author information

1
Molecular Virology Laboratory, POSTECH Biotech Center, Department of Life Science, Pohang University of Science and Technology, Pohang, Korea.

Abstract

MicroRNAs (miRNAs) are small noncoding RNAs that mediate post-transcriptional gene silencing by binding to complementary target mRNAs and recruiting the miRNA-containing ribonucleoprotein complexes to the mRNAs. However, the molecular basis of this silencing is unclear. Here, we show that human Ago2 associates with the cap-binding protein complex and this association is mediated by human eIF4GI, a scaffold protein required for the translation initiation. Using a cap photo-crosslinking method, we show that Ago2 closely associates with the cap structure. Taken together, these data suggest that eIF4GI participates in the miRNA-mediated post-transcriptional gene silencing by promoting the association of Ago2 with the cap-binding complex.

PMID:
23409027
PMCID:
PMC3567085
DOI:
10.1371/journal.pone.0055725
[Indexed for MEDLINE]
Free PMC Article

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