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Obesity (Silver Spring). 2013 Nov;21(11):2342-9. doi: 10.1002/oby.20341. Epub 2013 May 25.

Markers of macrophage infiltration and measures of lipolysis in human abdominal adipose tissues.

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1
Endocrinology and Genomics, Laval University Medical Center, 2705 Laurier Blvd. (T3-67), Québec G1V 4G2, Canada; Department of Nutrition, Laval University, Pavillon Paul Comtois, Québec G1K 7P4, Canada.

Abstract

OBJECTIVE:

We tested the hypothesis that high lipolytic responsiveness is related to increased expression of ATM genes in human adipose tissues.

DESIGN AND METHODS:

Omental (OM) and subcutaneous (SC) fat samples were obtained surgically in 46 women (age: 47.2 ± 4.7 years, BMI: 26.9 ± 5.2 kg/m(2) ). Body composition and fat distribution were measured using dual energy X-ray absorptiometry and computed tomography. Lipolysis was measured by glycerol release in mature adipocytes isolated by collagenase digestion under basal-, isoproterenol (10(-5) M)-, and forskolin (10(-5) M)-stimulated conditions. Quantification of macrophage gene mRNA expression (CD11b, CD11c, and CD68) in whole adipose tissue was performed using real-time RT-PCR.

RESULTS:

SC CD68 mRNA abundance was positively associated with isoproterenol-stimulated lipolysis (r = 0.36, P < 0.05). This association remained significant after adjustment for total body fat mass (r = 0.34, P ≤ 0.05). In the OM depot, CD11b mRNA abundance was positively associated with isoproterenol-stimulated lipolysis (r = 0.42, P ≤ 0.005). This association remained significant after adjustment for total body fat mass (r = 0.41, P ≤ 0.01). In subgroup analyses, high lipolytic rates in SC adipocytes were related to increased whole tissue expression of CD68 and CD11b in this compartment, independent of adiposity and fat cell size (P ≤ 0.001 and P ≤ 0.05). High lipolytic rates in OM adipocytes were related to increased whole tissue OM expression of CD11b, independent of adiposity and fat cell size (P ≤ 0.05).

CONCLUSIONS:

High adipocyte lipolytic responsiveness is related to increased expression of ATM markers in the corresponding compartment, independent of adiposity and fat cell size.

PMID:
23408706
DOI:
10.1002/oby.20341
[Indexed for MEDLINE]
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