Send to

Choose Destination
See comment in PubMed Commons below
Nucleic Acid Ther. 2013 Apr;23(2):131-9. doi: 10.1089/nat.2012.0403. Epub 2013 Feb 13.

Evaluation of biodegradable peptide carriers for light-directed targeting.

Author information

  • 1Department of Tumor Biology, Institute for Cancer Research , The Norwegian Radium Hospital, Oslo, Norway .


A promising strategy for increased intracellular delivery of nucleic acids with the benefit for targeting is photochemical internalization (PCI). PCI relies on the use of a photosensitizing compound that photochemically destroys membranes in the endocytic pathway after illumination, resulting in cytosolic transfer of endosomal content. PCI technology combined with biodegradable polyamino acid carriers and nucleic acids delivers effective targeting and improved biosafety. In an in vitro model system, we have evaluated various poly-l-lysine (PLL), poly-l-histidine (PLH), and poly-l-arginine (PLA) formulations for light-directed small interference RNA (siRNA) gene silencing and messenger RNA (mRNA) delivery. We find that PLA formulations are suitable as siRNA and mRNA carriers in a strictly light-directed manner.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Atypon
    Loading ...
    Support Center