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Asia Pac Allergy. 2013 Jan;3(1):50-8. doi: 10.5415/apallergy.2013.3.1.50. Epub 2013 Jan 30.

Murine subcutaneous immunotherapy models with beneficial immunological and physiological effects.

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1
Department of Internal Medicine, Seoul National University College of Medicine, Seoul National University Medical Research Center, Seoul 110-744, Korea. ; Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, Seoul 110-744, Korea. ; Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam 463-802, Korea.

Abstract

BACKGROUND:

Immunotherapy was introduced 100 years ago and has a unique role in the treatment of allergic diseases in that only immunotherapy can induce long-term immunological tolerance. However, only a few mouse models of immunotherapy have been developed so far.

OBJECTIVE:

We tried to establish murine immunotherapy models that have similar findings in human using subcutaneous rush immunotherapy-like schedule.

METHODS:

To determine the maximal safe or maximal tolerable dose, injection dose was doubled twice a day from the dose of sensitization. Mice with established asthma using ovalbumin (OVA) were repeatedly injected with OVA from the dose of sensitization subcutaneously twice a day: after reaching to the maximal safe or maximal tolerable dose, mice were injected with each dose either 10 times or 24 times.

RESULTS:

Short term immunotherapy (10 times) with the maximal safe and tolerable dose of OVA showed decreased IL-5 production, decreased IL-5/INF-γ ratio, and increased IgG2a/IgG1 but there was no significant difference in airway hyperresponsiveness (AHR) or airway inflammation. Prolonged immunotherapy (24 times) with the maximal tolerable dose not only decreased cytokine productions of IL-5 and even INF-γ, but also decreased IgE, IgG1 and even IgG2a production. Remarkably, the prolonged immunotherapy provided a protective effect on AHR.

CONCLUSION:

This study suggested immunotherapy models with some beneficial immunological and physiological effects in murine asthma.

KEYWORDS:

Allergy; Animal models; Asthma; Immunotherapy

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