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EMBO J. 2013 Mar 20;32(6):886-98. doi: 10.1038/emboj.2013.23. Epub 2013 Feb 12.

Signal recognition initiates reorganization of the presequence translocase during protein import.

Author information

1
Department of Biochemistry II, University of Göttingen, Göttingen, Germany.

Abstract

The mitochondrial presequence translocase interacts with presequence-containing precursors at the intermembrane space (IMS) side of the inner membrane to mediate their translocation into the matrix. Little is known as too how these matrix-targeting signals activate the translocase in order to initiate precursor transport. Therefore, we analysed how signal recognition by the presequence translocase initiates reorganization among Tim-proteins during import. Our analyses revealed that the presequence receptor Tim50 interacts with Tim21 in a signal-sensitive manner in a process that involves the IMS-domain of the Tim23 channel. The signal-driven release of Tim21 from Tim50 promotes recruitment of Pam17 and thus triggers formation of the motor-associated form of the TIM23 complex required for matrix transport.

PMID:
23403928
PMCID:
PMC3604718
DOI:
10.1038/emboj.2013.23
[Indexed for MEDLINE]
Free PMC Article

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