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J Microbiol Methods. 2013 Apr;93(1):49-51. doi: 10.1016/j.mimet.2013.01.021. Epub 2013 Feb 10.

Tryptic soy medium is feasible for the in situ preparation of standards containing small defined numbers of microbial cells.

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1
Tokyo University of Agriculture and Technology, Department of Biotechnology and Life Science, 2-24-16 Naka-cho, Koganei, Tokyo, Japan. mhide@cc.tuat.ac.jp

Abstract

A standard material comprising a few viable cells of a microorganism is essential for rational validation of microbiological methods. We propose a method of a flow cytometric sorting of cells stained with 6-carboxyfluorescein diacetate. The feasibility of tryptic soy medium in this method is demonstrated with 5 strains.

PMID:
23403310
DOI:
10.1016/j.mimet.2013.01.021
[Indexed for MEDLINE]
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