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Biochim Biophys Acta. 1990 May 8;1038(3):295-9.

L-pipecolic acid metabolism in human liver: detection of L-pipecolate oxidase and identification of its reaction product.

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Department of Biochemistry, University of Maryland Dental School, Baltimore 21201.


L-Pipecolate oxidase, an enzyme that oxidizes L-pipecolic acid in the human liver has been demonstrated in the peroxisomal preparation. This enzyme oxidizes L-pipecolic acid with concomitant production of H2O2 in the peroxisome of the normal human liver. The immediate product of L-pipecolic acid oxidation has been identified as L-alpha-aminoadipate delta-semialdehyde. This reaction product was directly, and also after conversion to pipecolic acid by NaBH4 reduction, characterized by use of an amino acid analyzer and thin-layer chromatography. The pit fall of an indirect assay of L-pipecolate oxidase by means of the assay of alpha-aminoadipic acid formation was discussed.

[Indexed for MEDLINE]

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