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Biosens Bioelectron. 2013 Jun 15;44:115-21. doi: 10.1016/j.bios.2012.12.046. Epub 2013 Jan 2.

Detection of exosomal biomarker by electric field-induced release and measurement (EFIRM).

Author information

1
UCLA School of Dentistry, UCLA Dental Research Institute, University of California, Los Angeles, CA 90095-1668, USA. frada@ucla.edu

Abstract

Exosomes biomarkers mediating important biological process, especially in the systemic disease diagnostics and therapeutics, yet the protective exosomal vesicle structure hinders rapid, simple detection of the harbored molecules. We have established a new method, the electric field-induced release and measurement (EFIRM), which can simultaneously disrupt exosomes to release the contents and on-site monitoring the harbored exosomal RNA/proteins biomarkers. When exposed to a non-uniform electrical field, exosomal RNA and proteins are rapidly released. Bio-recognition of these biomolecules is carried out concurrently. We tested the hypothesis that the lung cancer cell line, H460 stably transfected with hCD63-GFP, would shed hCD63-GFP expressing exosomes that could be detected in serum and saliva. We confirmed in vivo that H460-CD63-GFP shed exosomes were transported to blood and saliva. This result demonstrates for the first time tumor-shed exosomes were detected in saliva, in addition to blood, presenting a new translational utility of exosome-based biomarker detection in saliva.

PMID:
23402739
PMCID:
PMC3809103
DOI:
10.1016/j.bios.2012.12.046
[Indexed for MEDLINE]
Free PMC Article

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