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Carbohydr Res. 2013 Mar 22;369:48-53. doi: 10.1016/j.carres.2012.12.024. Epub 2013 Jan 16.

Structural characterization of α-galactosylated O-glycans from miniature pig kidney and endothelial cells.

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1
School of Chemical and Biological Engineering in College of Engineering, Seoul National University, Shillim-dong, Seoul 151-742, Republic of Korea.

Abstract

O-Glycan is a major oligosaccharide expressed on the surface of eukaryotic cells that plays a role in immune responses via cell-protein and cell-cell interaction. Unlike N-glycan and glycolipid-derived glycan, O-glycans have not been previously reported in pig organs. Here, we report the first instance of the identification of α1,3 galactosylated O-glycans from pig kidney and endothelial cells by using MALDI-TOF MS and nano-ESI MS/MS. The MALDI profile showed 28 O-glycans from miniature pig kidney and 18 O-glycans from pig endothelial cells. Among them, three potential α-galactosylated glycans considered as immunogenic antigens were identified by CID using ESI-MS/MS; Hexα1,3Hex-HexNAc-HexNAc, Hexα1,3Hex-Hex-HexNAc-HexNAc, and Hexα1,3Hex-Hex-Hex-Hex-HexNAc. This structural information on glycans will provide significant information to clinical studies regarding immune rejection responses induced by carbohydrate antigens during xenotransplantation.

PMID:
23399746
DOI:
10.1016/j.carres.2012.12.024
[Indexed for MEDLINE]
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