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Bioresour Technol. 2013 Mar;132:171-7. doi: 10.1016/j.biortech.2012.12.017. Epub 2012 Dec 14.

Novel bioconversions of municipal effluent and CO₂ into protein riched Chlorella vulgaris biomass.

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The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, PR China.


Batch, modified semi-continuous and continuous cultivations of Chlorella vulgaris C9-JN 2010 cells in municipal effluent were performed and analyzed. The experiments were carried out in 7.5-L photo-bioreactors, to which 2% of CO2 was supplied. Biomass and specific growth rate of C. vulgaris were 0.528-0.760gl(-1) and 0.200-0.374d(-1), respectively. Meanwhile, it could efficiently remove ammonia-N, total nitrogen, total phosphorus, CODCr and BOD5 by around 98.0%, 90.9-93.6%, 89.9-91.8%, 60.7-90.0% and 83.4-88.4%, respectively. Algal protein content was 550±30.0mgg(-1) of the harvested biomass of C. vulgaris which was rich in eight kinds of essential amino acids (around 44.5% of the total). The processes of cultivation of C. vulgaris in municipal effluent could be proposed as dual-beneficial approaches, which could produce profitable byproducts and simultaneously reduce the contaminations to environment.

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