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J Fluoresc. 2013 May;23(3):495-501. doi: 10.1007/s10895-013-1169-y. Epub 2013 Feb 10.

Rhodamine-based Cu2+-selective fluorosensor: synthesis, mechanism, and application in living cells.

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1
Department of Chemistry, National Institute of Technology, Durgapur, WB, India.

Abstract

A rhodamine B-based fluorescence probe (1) for the sensitive and selective detection of Cu(2+) ion has been designed and synthesized using pyridine moiety. The optical properties of this compound have been investigated in acetonitrile-water binary solution (7:3 v/v). Compound 1 is found to be an excellent sensor for a biologically/physiologically very important transition metal ion (Cu(2+)) using only the two very different modes of measurements (absorption and emission); one case displayed intensity enhancement whereas in other case showed intensity depletion (quenching). A mechanistic investigation has been performed to explore the static nature of quenching process. The sensor has been found to be very effective in sensing Cu(2+) ion inside living cells also.

PMID:
23397488
DOI:
10.1007/s10895-013-1169-y
[Indexed for MEDLINE]
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