Format

Send to

Choose Destination
Methods Mol Biol. 2013;979:65-70. doi: 10.1007/978-1-62703-290-2_7.

Detection of necrosis by release of lactate dehydrogenase activity.

Author information

1
Immunology and Virology Program, Department of Pathology, Center for AIDS Research, University of Massachusetts Medical School, Worcester, MA, USA. Francis.Chan@umassmed.edu

Abstract

Apoptosis and necrosis are two major forms of cell death observed in normal and disease pathologies. Although there are many assays for detection of apoptosis, relatively few assays are available for measuring necrosis. A key signature for necrotic cells is the permeabilization of the plasma membrane. This event can be quantified in tissue culture settings by measuring the release of the intracellular enzyme lactate dehydrogenase (LDH). When combined with other methods, measuring LDH release is a useful method for the detection of necrosis. In this chapter, we describe the step-by-step procedure for detection of LDH release from necrotic cells using a microtiter plate-based colorimetric absorbance assay.

PMID:
23397389
PMCID:
PMC3763497
DOI:
10.1007/978-1-62703-290-2_7
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Springer Icon for PubMed Central
Loading ...
Support Center