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Nat Methods. 2013 Mar;10(3):243-5. doi: 10.1038/nmeth.2366. Epub 2013 Feb 10.

Robust, synergistic regulation of human gene expression using TALE activators.

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1
Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, Massachusetts, USA.

Abstract

Artificial activators designed using transcription activator-like effector (TALE) technology have broad utility, but previous studies suggest that these monomeric proteins often exhibit low activities. Here we demonstrate that TALE activators can robustly function individually or in synergistic combinations to increase expression of endogenous human genes over wide dynamic ranges. These findings will encourage applications of TALE activators for research and therapy, and guide design of monomeric TALE-based fusion proteins.

PMID:
23396285
PMCID:
PMC3584229
DOI:
10.1038/nmeth.2366
[Indexed for MEDLINE]
Free PMC Article
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