Format

Send to

Choose Destination
FEBS Lett. 2013 Mar 18;587(6):763-9. doi: 10.1016/j.febslet.2013.01.068. Epub 2013 Feb 8.

Activation of beta 1 but not beta 3 integrin increases cell traction forces.

Author information

1
Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, USA. grlin@mail.med.upenn.edu

Abstract

Cell-generated traction forces induce integrin activation, leading to focal adhesion growth and cell spreading. It remains unknown, however, whether integrin activation feeds back to impact the generation of cytoskeletal tension. Here, we used elastomeric micropost arrays to measure cellular traction forces in wildtype and integrin-null cells. We report that activation of β1 but not β3 integrin, by either increasing density of immobilized fibronectin or treating with manganese, elicited fibroblast spreading and cytoskeletal tension. Furthermore, this force generation required Rho kinase and myosin activity. These findings suggest that integrin activation and cell traction forces comprise a bi-directional signaling unit of cell adhesion.

PMID:
23395612
PMCID:
PMC3966909
DOI:
10.1016/j.febslet.2013.01.068
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center