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J Agric Food Chem. 2013 Mar 13;61(10):2426-33. doi: 10.1021/jf4004048. Epub 2013 Feb 27.

Phage-displayed peptide that mimics aflatoxins and its application in immunoassay.

Author information

1
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences , Wuhan 430062, P. R. China.

Erratum in

  • J Agric Food Chem. 2013 Apr 24;61(16):3994.

Abstract

To search for an alternative to using protein conjugated aflatoxin as a coating antigen in aflatoxin detection by an ELISA method, a random-8-peptide library was constructed and used as a source of peptides that mimic aflatoxins (termed as mimotopes). Five mimotope peptides were obtained by panning-elution from the library and were successfully used in an indirect competitive ELISA for analyzing total aflatoxin concentration. The assay exhibited an IC50 value of 14 μg/kg in samples (with 1 in 7 dilution of sample extract) for aflatoxins. The linear range is 4-24 μg/kg. Further validation indicated relatively good recovery (60-120%) in peanut, rice and corn. Natural contaminated samples (peanut and feedstuff) were analyzed for aflatoxin concentration by both conventional ELISA and phage ELISA. The results showed good correlation. It can be concluded that the mimotope preparation is an effective substitute for the aflatoxin based coating antigen in ELISA and can be used in real sample analysis.

PMID:
23394544
PMCID:
PMC3830680
DOI:
10.1021/jf4004048
[Indexed for MEDLINE]
Free PMC Article

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