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Biochemistry. 2013 Feb 19;52(7):1131-3. doi: 10.1021/bi3015218. Epub 2013 Feb 7.

A yeast telomerase complex containing the Est1 recruitment protein is assembled early in the cell cycle.

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1
Salk Institute for Biological Studies, La Jolla, CA 92037, USA.

Erratum in

  • Biochemistry. 2013 Jul 23;52(29):5027.

Abstract

In budding yeast, association of the Est1 regulatory protein with telomerase is thought to be limited to the late S phase, when telomere elongation occurs. By monitoring the stoichiometry of telomerase subunits, we show instead that a telomerase complex containing Est1 is assembled much earlier in the cell cycle. We also report a biochemical interaction between Est1 and the telomere binding protein Cdc13 that recapitulates the previously observed genetic relationship between EST1 and CDC13. This supports a model in which regulated binding of Cdc13 to chromosome termini dictates subsequent interaction of a recruitment-competent telomerase complex with telomeres.

PMID:
23390975
PMCID:
PMC4172482
DOI:
10.1021/bi3015218
[Indexed for MEDLINE]
Free PMC Article
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