Format

Send to

Choose Destination
See comment in PubMed Commons below
PLoS One. 2013;8(2):e52960. doi: 10.1371/journal.pone.0052960. Epub 2013 Feb 4.

Characterization of a new series of fluorescent probes for imaging membrane order.

Author information

1
Centre for Vascular Research and Australian Centre for Nanomedicine, University of New South Wales, Sydney, Australia.

Abstract

Visualization and quantification of lipid order is an important tool in membrane biophysics and cell biology, but the availability of environmentally sensitive fluorescent membrane probes is limited. Here, we present the characterization of the novel fluorescent dyes PY3304, PY3174 and PY3184, whose fluorescence properties are sensitive to membrane lipid order. In artificial bilayers, the fluorescence emission spectra are red-shifted between the liquid-ordered and liquid-disordered phases. Using ratiometric imaging we demonstrate that the degree of membrane order can be quantitatively determined in artificial liposomes as well as live cells and intact, live zebrafish embryos. Finally, we show that the fluorescence lifetime of the dyes is also dependent on bilayer order. These probes expand the current palate of lipid order-sensing fluorophores affording greater flexibility in the excitation/emission wavelengths and possibly new opportunities in membrane biology.

PMID:
23390489
PMCID:
PMC3563652
DOI:
10.1371/journal.pone.0052960
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Public Library of Science Icon for PubMed Central
    Loading ...
    Support Center