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J Clin Microbiol. 2013 Apr;51(4):1301-2. doi: 10.1128/JCM.00028-13. Epub 2013 Feb 6.

Evaluation of PNA-FISH yeast traffic light for rapid identification of yeast directly from positive blood cultures and assessment of clinical impact.

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1
Department of Microbiology, Royal Free Hospital, London, United Kingdom. neil.stone@nhs.net

Abstract

The PNA-FISH Yeast Traffic Light assay was performed on 54 clinical isolates of yeasts inoculated into blood culture bottles. The assay showed high sensitivity (Candida albicans/C. parapsilosis, 100%; C. glabrata/C. krusei, 92.3%; C. tropicalis, 100%) and specificity (C. albicans/C. parapsilosis, 100%; C. glabrata/C. krusei, 94.8%; C. tropicalis, 100%). Case note review estimated a change in therapy in 29% of cases had the PNA-FISH result been available to the clinician.

PMID:
23390280
PMCID:
PMC3666762
DOI:
10.1128/JCM.00028-13
[Indexed for MEDLINE]
Free PMC Article
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