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Mult Scler. 2013 Aug;19(9):1137-44. doi: 10.1177/1352458513475489. Epub 2013 Feb 6.

An assay to quantify species-specific anti-JC virus antibody levels in MS patients.

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1
Department of Neurology, Medical Faculty, Heinrich-Heine-University, Germany.

Abstract

BACKGROUND:

The StratifyJCV® test is a qualitative assay to classify MS patients as anti-JC virus (JCV) antibody positive or negative. Quantification of anti-JCV antibody levels in serum and cerebrospinal fluid (CSF) of multiple sclerosis (MS) patients might add to the progressive multifocal leukoencephalopathy (PML) risk assessment.

OBJECTIVE:

The objective of this study is to test sera of patients in a quantitative anti-JCV antibody assay, and to compare the results with preexisting data from the StratifyJCV® test.

METHODS:

Sera of a total of 175 MS patients and matched non-MS-controls were tested for anti-JCV antibodies using glutathione S-transferase-tagged-VP1 as antigen. Antibody reactivity was quantified in arbitrary units using human immunoglobulin as standard.

RESULTS:

The comparison of our assay with StratifyJCV® showed good inter-assay agreement (kappa 0.6), and strong correlation for antibody reactivity (r (2) = 0.94). Discordant samples had low-reactive positivity, and a higher proportion (13% vs. 4%) tested positive in the StratifyJCV® test only.

CONCLUSIONS:

The method presented is a tool for the reliable quantification of anti-JCV antibodies, which demonstrates good agreement with results from StratifyJCV®. In contrast to StratifyJCV®, we pre-adsorbed all of the sera with BK virus (BKV) VP1 protein to reduce cross-reactivity. This step may account for a higher species-specificity of our assay. As such, our assay might be a promising additional tool for PML risk assessment.

KEYWORDS:

BK virus; JC virus; Multiple sclerosis; Tysabri; progressive multifocal leukoencephalopathy

PMID:
23388163
DOI:
10.1177/1352458513475489
[Indexed for MEDLINE]
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