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Methods Mol Biol. 2013;975:71-81. doi: 10.1007/978-1-62703-278-0_6.

Virus-induced gene silencing in the rapid cycling columbine Aquilegia coerulea "Origami".

Author information

1
Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA.

Abstract

Aquilegia Origami is an emerging model system for ecology and evolution, which has numerous genetic and genomic tools. Virus-induced gene silencing (VIGS) has been established as an effective approach to study gene function in Aquilegia. In the current protocol, we demonstrate VIGS using Agrobacterium strain GV3101 carrying tobacco rattle virus (TRV)-based constructs to infect Aquilegia coerulea "Origami" plants via vacuum infiltration.

PMID:
23386296
DOI:
10.1007/978-1-62703-278-0_6
[Indexed for MEDLINE]

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