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Cold Spring Harb Protoc. 2013 Feb 1;2013(2):118-21. doi: 10.1101/pdb.top072900.

An optical fiber-based uncaging system.

Abstract

This article describes the assembly and performance of a simple and inexpensive ultraviolet-flash system suitable for rapid focal photolysis of caged compounds in cultured neurons and brain slices. Advantages and limitations of this system are discussed. Examples are provided illustrating how this system can be used for stimulating neurons and mapping their functional inputs in brain slices.

PMID:
23378654
PMCID:
PMC3734551
DOI:
10.1101/pdb.top072900
[Indexed for MEDLINE]
Free PMC Article

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