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Nat Methods. 2013 Mar;10(3):256-8. doi: 10.1038/nmeth.2362. Epub 2013 Feb 3.

Generating parabiotic zebrafish embryos for cell migration and homing studies.

Author information

1
Institut Pasteur, Unité Macrophages et Développement de l'Immunité, Département de Biologie du Développement et Cellules Souches, Paris, France.

Abstract

Parabiosis, the surgical generation of conjoined organisms sharing a common bloodstream, has been a powerful tool for studying hematopoietic cell migration and interaction with stromal niches in rodent and avian systems. We describe a technique to generate parabiotic zebrafish embryos based on blastula fusion. This procedure permits the in vivo visualization of hematopoietic cell migration and homing to niches and peripheral tissues in zebrafish parabiotes of different genetic backgrounds.

PMID:
23377378
DOI:
10.1038/nmeth.2362
[Indexed for MEDLINE]

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